Environmental Disease

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 4  |  Issue : 3  |  Page : 62--68

PM2.5inhalation aggravates inflammation, oxidative stress, and apoptosis in nonalcoholic fatty liver disease


Shen Xin1, Jing Qu2, Na Xu2, Baohong Xu1 
1 Department of Gastroenterology, Beijing Luhe Hospital, Capital Medical University, Beijing, China
2 Department of Geriatric Medicine, Beijing Luhe Hospital, Capital Medical University, Beijing, China

Correspondence Address:
Dr. Baohong Xu
Department of Gastroenterology, Beijing Luhe Hospital, Capital Medical University, Beijing 101149
China

Background: Particulate matter under 2.5 μm (PM2.5) is a major risk factor for nonalcoholic fatty liver disease (NAFLD). This study aimed to investigate whether PM2.5could aggravate NAFLD, as well as its relative mechanisms. Materials and Methods: Male Sprague-Dawley rats were under PM2.5exposure and filtered air with NAFLD for 4, 6, and 8 weeks. Blood lipids were measured by enzyme-linked immunosorbent assay (ELISA). The histopathology of liver was determined by hematoxylin and eosin staining. ELISA assay was conducted for detecting inflammatory markers including interleukin-17 (IL-17) or tumor necrosis factor alpha (TNF-α) and for assessing oxidative stress-associated proteins, including superoxide dismutase (SOD) and malondialdehyde (MDA). Apoptosis was assessed by detecting B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (BAX) by real-time polymerase chain reaction and Western blotting. Results: PM2.5exposure for 8 weeks, but not 4 or 6 weeks, significantly aggravated NAFLD, which was associated with the enhanced expression of IL-17 and TNF-α and the enhanced oxidative stress (SOD and MDA). Meanwhile, exposure PM2.5for 8 weeks, but not 4 or 6 weeks, regulated apoptosis (Bcl-2 and BAX). Conclusions: Exposure of PM2.5for 8 weeks can aggravate NAFLD, which may be mediated by liver inflammation, oxidative stress, and apoptosis.


How to cite this article:
Xin S, Qu J, Xu N, Xu B. PM2.5inhalation aggravates inflammation, oxidative stress, and apoptosis in nonalcoholic fatty liver disease.Environ Dis 2019;4:62-68


How to cite this URL:
Xin S, Qu J, Xu N, Xu B. PM2.5inhalation aggravates inflammation, oxidative stress, and apoptosis in nonalcoholic fatty liver disease. Environ Dis [serial online] 2019 [cited 2023 Feb 1 ];4:62-68
Available from: http://www.environmentmed.org/article.asp?issn=2468-5690;year=2019;volume=4;issue=3;spage=62;epage=68;aulast=Xin;type=0